5' scRNAseq of splenic CD3+ cells from immunodeficient mice injected with progenitor T-cells generated from naïve, non-expanded or SR1-expanded human cord blood-derived CD34+ cells

We report the gene expression profile of single cell peripheral CD3+ cells from immunodeficient mice injected with human progenitor T-cells (proT-cells). ProT-cell subsets were generated in vitro using human umbilical cord blood-derived CD34+ cells that were either non-expanded (naive), or expanded in vitro with the hydrocarbon receptor antagonist, StemRegenin-1 (SR1). Overall design: Splenic CD3+ T-cells were FACS sorted from immunodeficient mice injected with naive progenitor T-cells (generated from non-expanded CD34+ cells) or SR1 progenitor T-cells (generated from StemRegenin-1-expanded CD34+ cells).