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Construction of a Bioluminescent Reporter Strain To Detect Polychlorinated Biphenyls

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PubMed Central2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC90961/
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资源简介:
A bioluminescent reporter strain, Ralstonia eutropha ENV307(pUTK60), was constructed for the detection of polychlorinated biphenyls by inserting the biphenyl promoter upstream of the bioluminescence genes. In the presence of a nonionic surfactant, which enhances the solubility of chlorinated biphenyls, bioluminescence was induced three- to fourfold over background by biphenyl, monochlorinated biphenyls, and Aroclor 1242. The minimum detection limits for these compounds ranged from 0.15 mg/liter for 4-chlorobiphenyl to 1.5 mg/liter for Aroclor 1242.
提供机构:
American Society for Microbiology (ASM)
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