WGS & Clone-based genome sequencing. Eremothecium gossypii ATCC 10895

The Biozentrum, University of Basel, Switzerland, in collaboration with Syngenta, completed the sequencing of the Eremothecium gossypii (aka Ashbya gossypii) strain ATCC 10895 nuclear and mitochondrial genomes in 2001. The nuclear genome is sequenced at approximately 4.2X coverage; regions of low quality are identified. This sequencing project was initiated when a high level of conservation of synteny between Eremothecium gossypii and Saccharomyces cerevisiae, which diverged from each other more than 100 million years ago, was discovered. Annotation of the Eremothecium gossypii genome revealed 4,718 protein-coding genes in the nuclear genome and eight in the mitochondrial genome. Over 90% of the Eremothecium gossypii genes share both homology and synteny with Saccharomyces cerevisiae genes. The majority of the Eremothecium gossypii genes occur in duplicate in Saccharomyces cerevisiae, which is considered to be evidence that the evolution of Saccharomyces cerevisiae included either a whole-genome duplication or a fusion of two related species.