Cooperative ETS Transcription Factors Enforce Adult Endothelial Cell Fate and Cardiovascular Homeostasis [Liver_IL1B_TNFA_RNAseq]

Bulk RNA-seq of mouse in in vitro samples from either control or Erg and Fli1 knock out with or without additional treatment of Il1b + Tnfa Overall design: Mouse ECs were isolated from the liver of mice with the corresponding genotype. The induction of the deletion of Erg and/or Fli1 in cultured ECs was achieved by the addition of 4-OHT for 48 hours to the culture medium. Cells were cultured for an additional 7 days after the initial addition of tamoxifen before final harvest and analysis. To study the inflammatory response of the ECs, we added both IL1ß and TNFa at day 7 after tamoxifen administration and lysed the cells 16 hours afterwards for RNA-seq analysis.