Additional file 2: Figure S2. of Conservation of estrogen receptor function in invertebrate reproduction

Six fluorescent synthetic ligands (AMIs) were tested for in vivo binding to ER in live rotifer neonates and with the yeast chemical complementation assay using a cloned rotifer ER LBD. These compounds were chosen because previous work verified their binding to the human ER LBD in yeast chemical complementation assays. These compounds fluoresce upon binding to their target [35]. Estradiol is the natural ligand for the human ER and does not fluoresce. (XLS 47 kb)