Cecal microbiome of EPEC-challenged C57BL/6 mice under feline-derived Ligilactobacillus supplementation

This BioProject contains 16S rRNA gene (V3-V4) amplicon sequencing data generated from cecal contents of male C57BL/6 mice in an enteropathogenic Escherichia coli (EPEC) challenge model. Mice were assigned to six groups: control, EPEC model, chlortetracycline positive control, probiotic treatment, probiotic pre-treatment, and continuous probiotic supplementation. The probiotic consisted of a 1:1 mixture of feline-derived Ligilactobacillus agilis ZY25 and Ligilactobacillus salivarius ZY35 administered according to the assigned regimen. Amplicons were generated using primers 338F and 806R and sequenced on an Illumina MiSeq PE300 platform. The dataset supports analyses of how probiotic timing modulates EPEC-associated intestinal injury and cecal microbial community structure.