RNA-seq profiling of METTL1 knockdown in human intestinal epithelial FHC cells

METTL1 is a key methyltransferase responsible for N7-methylguanosine (m7G) modification of RNA and has been implicated in multiple cellular processes. However, its role in human intestinal epithelial cells remains incompletely understood. In this study, we performed RNA sequencing (RNA-seq) to characterize transcriptomic changes induced by METTL1 knockdown in human fetal intestinal epithelial (FHC) cells. Stable METTL1 knockdown was achieved using lentiviral short hairpin RNA (shRNA), while control cells were infected with a non-targeting control shRNA lentivirus (sh-Ctrl). Knockdown efficiency was validated prior to sequencing. Total RNA was extracted from METTL1-knockdown and sh-Ctrl FHC cells, with three independent biological replicates per group. This dataset provides a transcriptomic resource for investigating the regulatory role of METTL1 in intestinal epithelial cells. Overall design: Human fetal intestinal epithelial FHC cells were divided into two groups: METTL1 knockdown and shRNA control. Lentiviral shRNA targeting METTL1 (sh-METTL1) was used to stably suppress METTL1 expression in FHC cells, while control cells were infected with a non-targeting control shRNA lentivirus (sh-Ctrl). After confirmation of knockdown efficiency, total RNA was extracted and subjected to high-throughput RNA sequencing. Each group consisted of three independent biological replicates. Differential gene expression analysis was performed to identify transcriptional changes associated with METTL1 depletion.