Caenorhabditis elegans Temperature-Sensitive Mutant Collection Genome sequencing

A collection of whole-genome shotgun sequenced Caenorhabditis elegans strains isolated from 3 diverse temperature-sensitive lethal mutant screens. Samples have been sequenced to a minimum 12-fold coverage. We hope to publish sequence data on approximately 300 strains total. For sequencing we load size-selected, barcoded samples on a Hi-Seq sequencing machine (Illumina) and do paired-end reads. Data analysis employs phaster, SamTools and custom filters.