RSPB-2020-0112-Microbiome Pooled Data OA

This dataset represents experiments exploring the role of <i>Galleria mellonella </i>microbiome in polyethylene degradation.<i> </i>1- Glycol production: <i>Galleria mellonella</i> larvae<i> </i>were treated with broad spectrum antibiotic and fed polyethylene or their honeycomb diet, and we monitored glycol excretion as a metabolic by-product of polyethylene degradation. 2-Antibiotic effect: Using qPCR we established the relative microbial abundance in <i>Galleria mellonella</i> after 24 hours<i>. </i>3-Excreta and Bacterial Abundance: Using qPCR we quantified microbial abundance (16S) of <i>Galleria mellonella</i> fed on polyethylene that excreted vs. did not excrete glycol. 4-Feeding Bacterial Abundance: We monitored bacterial abundance in waxworms under 3 feeding regimes: honeycomb, polyethylene and starvation for 24 and 72h.