Rapid preparation of electrocompetent Listeria monocytogenes and enhancement of transformation efficiency with cAMP supplementation

Background Listeria monocytogenes is a significant foodborne pathogen associated with serious health risks. The ability to genetically manipulate this bacterium is critical for understanding its pathogenic mechanisms and developing new interventions. However, low transformation efficiency and the absence of natural competence in L. monocytogenes present challenges for genetic studies. Genome assemblies Reads for isolates BL86-016 and BL87-009 are also available at BioProject PRJNA837734. Draft assemblies of these two isolates were constructed using shovill v1.1.0 with SPAdes v3.14 selected as assembler from quality trimmed reads generated using TrimGalore v0.4.3. Genomes were annotated using Bakta v1.6.1 Files File BL87_009.fasta corresponds to the draft assembly of L. monocytogenes isolate BL87-009 and annotations are in file BL87-009_257.gbk File BL86_016.fasta corresponds to the draft assembly of L. monocytogenes isolate BL87-009 and annotations are in file BL86-016.gbk File 02-5993.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 02-5993File 07-029_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 07-029File 07-030_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 07-030File 07-031_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 07-031File 07-036_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 07-036File 07-039_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 07-039File 08-5578.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 08-5578File 08-6056.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 08-6056File 10-0809.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 10-0809File 10-0812.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 10-0812File 10-0933.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 10-0933File 10-4754.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 10-4754File 10-4758.gbk corresponds to the annotation of the assembly of L. monocytogenes isolate 10-4758File 88-1059_draft.gbk corresponds to the annotation of the draft assembly of L. monocytogenes isolate 88-1059File BL86-009_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL86-009File BL86-010_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL86-010File BL86-012_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL86-012File BL87-014_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-014File BL87-015_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-015File BL87-016_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-016File BL87-019_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-019File BL87-025_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-025File BL87-028_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-028-BFile BL87-035_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-035File BL87-036_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-036File BL87-038_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-038File BL87-039_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-039File BL87-056_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL87-056File BL88-004_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-004File BL88-005_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-005File BL88-013_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-013File BL88-014_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-014File BL88-015_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-015File BL88-019_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-019File BL88-020_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-020File BL88-021_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-021File BL88-023-B_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-023-BFile BL88-029_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-029File BL88-030_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-030File BL88-034_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-034File BL88-071_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-071File BL88-072_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-072File BL88-081_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-081File BL88-097_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL88-097File BL89-006_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL89-006File BL89-010_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL89-010File BL89-012-B_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL89-012-BFile BL89-013_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL89-013File BL89-023_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL89-023File BL89-035_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL89-035File BL91-018_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL91-018File BL91-019_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL91-019File BL91-023_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL91-023File BL91-024_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL91-024File BL91-025_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL91-025File BL94-005_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate BL94-005File SRR7163869_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 399454File SRR7167591_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 241745File SRR7850130_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 246242File SRR7866629_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 396044File SRR7873592_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 246255File SRR7873684_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 259390File SRR9226492_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 754322File SRR9298670_pilon.gbk corresponds to the annotation of the hybrid assembly of L. monocytogenes isolate 757923