Transcriptomic analyses of ChREBPtotal-null mice and ChREBP-null mice liver

The Carbohydrate Responive Element Binding Protein (ChREBP) is a transcription factor sensing glucose. Two isoforms are produced by the same gene, the long isoform ChREBPalpha and the short one ChREBPbeta transcribed from an alternative promoter. ChREBPalpha activates transcription of ChREBPbeta in response to glucose. The latter is thought to mediate most of the ChREBP transcriptional activation of target genes. However, the contribution of ChREBPbeta in metabolic adaptations in vivo is not known. This study aims at dertermining the contribution of the short isoform ChREBPbeta to the changes in gene expression mediated by ChREBP. The study contains 4 groups of mice: total ChREBP knock-out mice, ChREBPbeta knock-out mice, and the respective control wild type littermates of the two transgenic lines.