qPCR data and analysis.

H9Δmat15q_1 is designated as AS-13, H9Δmat15q_2 is designated as AS-22, and H9Δpat15q is designated as PWS-2. Sheet titled “RT Set Up” details the experimental set up for the reverse transcriptase reaction to convert RNA samples to cDNA. Sheet titled “qPCR Set Up” details the calculations for performing qPCR experiments. Sheet titled “qPCR Data” contains all raw data from qPCR instrument. Sheet titled “Grubbs Test” contains calculations for outlier testing using Grubbs test. Sheet titled “StDev Calc” contains standard deviation calculations between technical replicates and across biological replicates for all samples; TechRep is short for technical replicate, BR is short for biological replicate. Sheet titled “Error Calculation” contains propagated error calculations, which includes technical and biological error. Sheet titled “qPCR Analysis (Cell Type)” contains relative expression calculations for all samples; in this case, ESC samples are compared to WT H9 ESCs which are set to a relative value of 1 and neuron samples are compared to WT H9 neurons which are set to a relative value of 1. Sheet titled “Statistics (Cell Type)” contains all statistics calculations per gene based on calculations performed in “qPCR Analysis (Cell Type).” Sheet titled “For R (Cell Type)” is to be saved as a.csv file and input into R for plotting; this was used to generate Figs 2 and 3B–3D. Sheet titled “qPCR Analysis (to WT ESCs)” contains relative expression calculations for all samples; in this case, all samples are compared to WT H9 ESCs which are set to a relative value of 1. Sheet titled “Statistics (to WT ESCs)” contains all statistics calculations per gene based on calculations performed in “qPCR Analysis (to WT ESCs).” Sheet titled “For R (to WT ESCs)” is to be saved as a.csv file and input into R for plotting; this was used to generate S7 Fig.
(XLSX)