Amplicon sequence of pathogenic genes. Enterobacteriaceae

We aimed to develop a method to simultaneously detect various pathogens by sequencing multiplex PCR amplicons, compare the results with those obtained by digital PCR, and apply the method to detect pathogens in river water and sewage samples. This data is the sequence of the pathogenic gene in the positive control sample amplified by multiplex PCR. It was used to discuss the relationship between gene copy number and read count.