Characterization of HEK293T-SPINDOC KO vs WT by RNA-Seq profiling

Background: SPIN1 is a known histone code effector protein, which is composed of three Tudor domains. With its second Tudor domain, it reads the H3K4me3 mark, which is the canonical signal for transcriptional activation. It also has the ability to recognize combinatorial marks like H3R8me2a and H3K9me3 through its first Tudor domain, when they occur in the presence of K3K4me3. We identified SPINDOC as a stable component of the SPIN1 protein complex. SPINDOC referred to its ability to dock with SPIN1, which was previously called C11orf84 before it was functionally deorphanized. Objective: To investigate the biological function of SPINDOC, we generated SPINDOC KO by CRISPR technology in HEK293T cell lines and performed RNA sequencing. Conclusion:Our data represent the first detailed analysis of SPINDOC associated transcriptome, with biologic replicates, generated by RNA-seq technology. Comprehensive RNA-seq analysis demonstrated that 1005 genes were differentially regulated, including 459 up-regulated genes and 546 down-regulated genes. Two pairs cell lines HEK29T-SPINDOC KO-1/WT-1 and HEK29T-SPINDOC-KO-2/WT-2