TfR1 globally regulates transcription and alternative splicing

In the current study, to figure out the regulation pattern of TfR1, we knocked down TFRC expression level by shRNA in HeLa cells. RNA-sequencing (RNA-seq) was used to analyze the global transcript level and alternative splicing (AS) on knockdown-treated (KD) and normal control (NC) cell samples. 629 differentially expressed genes (DEGs) were identified between OE and NC, and Gene ontology (GO) and KEGG analysis for DEGs were carried out. It was found that multiple DEGs were involved in O-glycan processing, protein modification, response to hypoxia and ATP catabolic process, indicating the down-regulated expression of TfR1 extensively disturbed cell physiology. To explore the regulation pattern of TfR1, we knocked down TFRC by shRNA in HeLa cells and RNA-sequencing (RNA-seq) was carried out in knockdown-treated (KD) versus normal control (NC) cell samples.