Total exRNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects

We sequenced small RNAs from 183 plasma samples, 204 urine samples and 46 saliva samples from 55 college athletes ages 18-25 years. Many of the participants provided more than one sample, weeks or months apart, allowing us to assess variability in an individual's exRNA expression levels over time. Several individuals provided all three biofluid types at one time, producing data on individual expression levels across several biofluid types. Here we provide a systematic analysis of small exRNAs present in each biofluid, as well as an analysis of exogenous RNAs. We find that a large number of RNA fragments in plasma (63%) and urine (54%) have sequences that are assigned to YRNA and tRNA fragments respectively. Surprisingly, while many miRNAs can be detected, there are few miRNAs that are consistently detected in all samples from a single biofluid. Additionally, we performed whole transcriptome sequencing on 134 plasma and 115 urine samples and identified circRNA.]]>
Inclusion Criteria Active Phase Participants A student athlete on the ASU football team that is on the active roster and participates in contact practice and games Willing to provide informed consent Willing to provide the required samples to the study Control Participants A student athlete who is a member of a non-contact or limited contact sports team and on the active roster Willing to provide informed consent Willing to provide the required samples to the study Exclusion Criteria History of neurologic disorders (such as epilepsy or cerebrovascular disease) ]]>
Study start date: June 2013]]>