P. pastoris fragment datasets

Results data of two-round SECRiFY screens of human (HEK293) cDNA fragments in P. pastoris (3 replicate screens). Sequencing data was mapped on the human GRCh38 transcriptome assembled using known transcripts from protein-coding genes only."Pp_resultstable_all.txt" = all in-frame fragments detected in either the unsorted baseline library (merged for 3 replicates), or in all 3 sorted replicate samples. "Pp_resultstable_enriched.txt" = those with log_FC > 1 in all three replicates (10404)"Pp_resultstable_depleted.txt" = those with log_FC For each fragment, the following information is provided:# Ensembl_geneID --> Ensembl gene ID# Ensembl_txID --> Ensembl transcript ID# tx_start --> Transcript start position on human genome GRCh38# tx_end --> Transcript end position on human genome GRCh38# chr --> chromosome ## gene_symbol --> official gene symbol# frag_start --> fragment start position on the transcript, 0-based # frag_stop --> fragment end position on the transcript, 0-based# cDNA --> DNA sequence of the fragment# protein --> translated AA sequence of the fragment in frame 1# IND_count --> raw count value in the baseline (unsorted) library (merged for 3 replicates), NAs replaced by 0.001# SORT(1)_count --> raw count value in the sorted sample replicate (1), NAs replaced by 0.001# IND_FPTM --> normalized FPTM value in the baseline (unsorted) library # SORT(1)_FPTM --> normalized FPTM value in the sorted sample replicate (1)# logFC_(1) --> log2(SORT(1)_FPTM/IND_FPTM)