RNA-seq analysis of vehicle and DOPPA-treated human cord blood CD34+ cells

In this study, we did RNA-sequencing analysis to check the transcriptome of vehicle and DOPPA-treated CD34+ cells after 4 days ex-vivo culture. We noticed that expression of many genes which encode cell surface proteins including ITGA6 (CD49f) were significantly up-regulated, FDR-adjusted p-value < 0.01 and the fold change (FC) > 2, by DOPPA treatment. Gene ontology (GO) analysis revealed that many GO functions related to cell surface or plasma membrane related GOs which all include ITGA6 were significantly enriched in up-regulated differentially expressed genes (DEGs) including ITGA6 after DOPPA treatment. These data suggest that DOPPA treatment promotes transcription of ITGA6. Overall design: Identification of the differentially expressed mRNA profiles in vehicle and DOPPA expanded human cord blood CD34+ cells.