Gut microbiota sequencing of mice with trauma

This study compares the gut microbiota composition between mice with trauma and mice supplemented with Akkermansia muciniphila using 16s rRNA sequencing. Male mice weighing 22 grams were used in the experiment. All mice were acclimatized for 14 days prior to treatment. The animals were randomly divided into two groups: a trauma control group and a trauma treatment group. In the first group, mice received blunt thoracic trauma and were administered 100 microliters of sterile PBS via oral gavage daily as the control treatment, referred to as Trauma PBS. In the second group, mice received blunt thoracic trauma and were administered 100 microliters of sterile AKK bacterial suspension via oral gavage daily as the treatment, referred to as Trauma AKK. Akkermansia muciniphila, also known as AKK, with the ATCC number BAA835, was purchased from Mingzhou Biotechnology in China.Blunt thoracic trauma was induced using a previously described mouse model of blunt chest injury. Under isoflurane gas anesthesia, a cylindrical weight weighing 50 grams was dropped from a height of 100 millimeters through a vertical stainless steel tube onto a platform placed on the chest of the mouse to induce blunt thoracic trauma. After the impact, the mice experienced transient shock, followed by rapid breathing, and regained motor function upon recovery from anesthesia.Mice were treated for 14 days post-trauma before sample collection. Fecal samples were collected using sterile containers and immediately frozen in an ultra-low temperature refrigerator. DNA was extracted using the Qiagen DNeasy PowerSoil Kit, and paired-end sequencing was performed on the Illumina MiSeq platform.