Climate change and urchin fertilisation and the effect on the growth rate of juvenile Abatus sp.

The effect of pH, temperature and sperm concentration on the fertilisation of Sterechinus neumayeri was investigated. Adult Sterechinus neumayeri were collected from Ellis Fjord Narrows between December and January 2011-12 and held in the Ecotox Field Aquarium Module until used. Between 3-4 male and female individuals were spawned using 0.5M KCl and gametes were collected separately before being fertilised in treatment. The data set shows the percentage of fertilised and non-fertilised eggs of Sterechinus neumayeri scored at 20h post-fertilisation. Eggs were fertilised in various combinations of pH, temperature and sperm concentration treatments (pH: 8.0 (Control), 7.8 and 7.6; Temperature: 1 degrees C (Control), 3 degrees C and 5 degrees C; Sperm concentration (sperm:egg ratio): 1000:1 (Control), 750:1, 250: 1, 50:1 and 5:1). At 20h post fertilisation, 5 ml aliquot was removed from fertilisation vials and eggs were counted and determined if they were fertilised or not. Seawater parameters of treatments were measured at the start and end of the experiment.Detailed information of the spreadsheets are as follows:Seawater Parameters column headings:Temperature - measured in degrees C , shows the temperature treatments usedpH - shows the pH levels usedSubheading pH - pH level measured for the day using NIST certified buffersSubheading MV - pH level measured for the day in millivoltsSubheading Total pH - total pH level in seawater obtained from MV measurementsSubheading Temp - temperature of seawater measured for the day1 deg C column headings:Experiment - number of experiments pH - shows the pH for each treatmentSperm Concentration - shows the sperm concentration used for each treatment in a egg:sperm ratioRep - shows the number of replicates per experimentUnfertilised eggs - eggs without visible fertilisation envelope and no cleavage after 20hFertilised eggs - eggs with visible fertilisation envelope and/or cleavage after 20hFertilised deformed eggs - eggs with visible fertilisation envelope but deformedTotal eggs - total eggs scored (whether fertilised or unfertilised)% Fertilised - fertilised eggs (deformed and non-deformed)/Total eggs3 deg C and 5 deg C have the same column headings as 1 deg C.AAS3134 Abatus sp Growth Experiment Davis 2011-12:The effect of pH and temperature on the growth rate of juvenile Abatus ingens and Abatus shackletoni were investigated. Adult Abatus were collected off Airport Beach in waters 4-5m depth. Data set shows the growth rate of juveniles of Abatus ingens and Abatus shackletoni after a 4-week exposure to various combinations of pH and temperature. Juveniles of each species was removed from maternal pouches and photographed on the oral side before being exposed to combinations of pH (8.0 (Control), 7.8 and 7.6) and temperature (-1 degrees C (Control) and 1 degrees C) levels. They were incubated in treatments for 4 weeks before being removed and rephotographed. The lengths of 10 spines per juvenile were measured in the pre- and post-experiment photographs using ImageJ and the difference calculated to get a growth rate per juvenile. Seawater parameters of treatments were measured at the beginning of the experiment and subsequently once a day until the end of the experiment.Detailed information of the spreadsheets are as follows:A ingens (pre-exp) i.e. juvenile Abatus ingens spine lengths measured before exposure to experimental treatments. Column headings are:Spine number and length (mm): Length of each spine (1 - 10) measured per juvenile in mm.R1 - R12: Number of juveniles A ingens (post-exp) i.e. juvenile Abatus ingens spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above.A shackletoni (pre-exp) i.e. juvenile Abatus shackletoni spine lengths measured before exposure to experimental treatments. Column headings are identical to the above.A shackletoni (post-exp) i.e. juvenile Abatus shackletoni spine lengths measured after 4-week exposure to experimental treatments. Column headings are identical to the above.2011-12 Aquarium pH and temp main headings show different treatment parameters. Column sub-headings are:Date - Date of measured seawater parametersSalinity - salinity of seawater measuredPpm - Amount of CO2 gas pumped into water recorded in parts per millionpH - measured pH of seawater using NIST-certified buffersMV - pH of seawater recorded in millivoltsTotal pH - total pH of seawater derived from MVTemp - Temperature of seawater measured in degrees C.