Single-cell analyses unravel cell type-specific responses to a vitamin D analogue in prostatic precancerous lesions [RNA-Seq]

We treated PTENpe-/- mice, in which PTEN is selectively ablated in prostatic epithelial cells, with a Vitamin D analog. We extracted RNA from the prostate of oil (vehicle) and Vitamin D analog-treated mice. Three conditions were investigated: control mice (PtenL2/L2), and Pten(i)pe-/- mice treated for three weeks with either a vehicle or the vitamin D analogue Gemini-72. Two biological replicates of control mice were prepared (n=2 prostates), whereas three biological replicates of each of the other two conditions were prepared (n=3 prostates/condition).