基于SSR标记的辽宁蒙古栎天然群体遗传多样性研究

采用10对SSR引物分析了8个天然群体共111个个体的遗传多样性。结果表明:每个SSR位点的平均等位基因数为7.3,平均有效等位基因数为2.813,平均期望杂合度为0.624,Shannon多样性指数为1.270。不同群体存在多样性差异,8个群体的期望杂合度在0.558～0.612,平均为0.588;群体分化系数（FST）为0.0655,表明遗传变异主要存在群体内,群体之间遗传分化较小;群体之间存在基因流（Nm）达到4.471,遗传距离和地理距离之间具有显著的正相关关系（r=0.631,p<0.05）。辽宁省分布的天然蒙古栎群体具有丰富的SSR多样性,其遗传多样性水平相对较高;群体间有较强的基因流,这导致不同群体间的遗传分化较低,绝大部分遗传变异是发生在群体内的,而且距离越远,遗传分化也越高。

A total of 111 individuals from 8 natural populations were assessed for genetic diversity using 10 pairs of Simple Sequence Repeat (SSR) primers. The results revealed that the mean number of alleles per SSR locus was 7.3, the average effective number of alleles was 2.813, the average expected heterozygosity was 0.624, and the Shannon's diversity index was 1.270. Significant variations in genetic diversity were detected across the 8 populations, with their expected heterozygosity ranging from 0.558 to 0.612 and an overall average of 0.588. The population fixation index (F_ST) was 0.0655, suggesting that the majority of genetic variation resided within populations, accompanied by low genetic differentiation among populations. The gene flow (Nm) between populations reached 4.471, and a significant positive correlation was observed between genetic distance and geographic distance (r=0.631, p<0.05). The natural Quercus mongolica populations distributed in Liaoning Province harbored abundant SSR-based genetic diversity with a relatively high level of genetic diversity. Strong gene flow among these populations contributed to low genetic differentiation between different groups, where the vast majority of genetic variation occurred within populations, and genetic differentiation increased with increasing geographic distance.

搜集汇总

背景与挑战

背景概述

该数据集基于SSR标记技术，研究了辽宁省8个天然蒙古栎群体的遗传多样性，共分析了111个个体。研究发现，蒙古栎群体具有较高的遗传多样性水平，遗传变异主要发生在群体内部，群体间分化较小且存在较强的基因流，遗传距离与地理距离呈显著正相关。数据集属于'北方主要珍贵用材树种高效培育技术研究'项目，提供了一份详细的研究报告文档。