Orangethroat darter, strawberry darter, current darter, brook darter, rainbow darter Raw sequence reads. Etheostoma spectabile, Etheostoma fragi, Etheostoma uniporum, Etheostoma burri, Etheostoma caeruleum

Double digest RADseq data were collected from 12 individuals from each of 5 species of darters. This data was used for population genetic analyses and to examine the relationship between genetic distance and behavioral isolation among species.