Single cell RNA-seq of mouse dorsal raphe Pet1 neurons

Here we use high-throughput and DR subdomain-targeted single-cell transcriptomics and intersectional genetic tools to map molecular and anatomical diversity of DR-Pet1 neurons. We describe up to fourteen neuron subtypes, many showing biased cell body distributions across the DR. Overall design: Single cell RNA-seq libraries prepared from genetically labeled Pet1 neurons in two different ways: (1) automated fluorescence-based cell sorting (using the On-Chip Sort) followed by 10X Genomics Chromium Single Cell 3' v3 protocol (samples 71-72), or (2) DR subdomain-targeted manual cell sorting, followed by SMART-Seq v4 Ultra Low Input cDNA amplification and Nextera XT library preparation (samples 1-70).