Random integration analysis in gene-edited iPS cell lines derived from Werner syndrome patients. Homo sapiens

Werner syndrome (WS), adult progeria, is characterized by accelerated-aging associated symptoms from a young age. The causative gene, WRN, is a RecQ DNA helicase and involved in DNA maintenance. The most prevalent mutational site is c.3139-1G>C, which is considered a founder mutation in Japan. We have corrected the gene of WS-iPS cells using CRISPR/Cas9 technology targeting the above mutation. Here, we show the results of random integration analysis in those cells.