Additional file 1: of Genome-wide methylation is modified by caloric restriction in Daphnia magna

Reads sequenced per replicate for both converted and unconverted libraries. Alignments analysed is number of read-pairs per bisulphite converted library aligned by Bismark; deduplicated is number of read pairs after removal of PCR duplicates; non CpG filtered is read-pairs remaining after removal of CHH and CHG containing reads; bases remaining are number of bases left for CpG methylation prediction in Bismark; average coverage is for filtered bases at a D. magna genome size of 240 megabases; CpG sites remaining before and after filtering of polymorphic sites, polymorphic sites were identified from unconverted libraries against a reference genome converted to Clone 32; Non CpG % methylated are CHH and CHG sites methylated (100 minus this figure is equivalent to bisulphite conversion efficiency), CpG % methylated is the genome-wide percentage of CpG methylation, and CpG % methylated filtered is the percentage remaining after reads containing CHH or CHG methylation are removed from the analysis. Diet, H: normal food, L: caloric restriction, code: combination of diet and mother. (XLSX 48 kb)