Comparative Analysis of RADseq Methods for SNP Discovery and Genetic Diversity Assessment in Safflower. Identification of suitable RAD seq approach and restriction enzyme combination for genotyping of safflower

This study aimed to optimize genotyping by sequencing methods for safflower using approaches with two RADseq strategies, single restriction site associated DNA sequencing and double-digest RAD sequencing and three restriction enzyme combinations, ApeKI, NlaIII and MseI, EcoRI and MseI.