DATASET S1: Transcriptomic profile of the cockle Cerastoderma edule exposed to Diarrhetic Shellfish Toxins seasonal contamination

Resulting .fasta and .gff files obtained from de novo transcriptome assembly and clustering analyses in the article: Transcriptomic profile of the cockle Cerastoderma edule exposed to Diarrhetic Shellfish Toxins seasonal contamination. Domínguez-Pérez, D. et al., 2021. Ce_assembly_unique.fasta: The de novo transcriptome assembly of C. edule before submission to NCBI, obtained with Trinity v2.10.0 by combining four samples (gills and digestive glands without DSTs and exposed) into a single transcriptome. C.edule_assembly_submitted_ncbi.fasta: The de novo transcriptome assembly submitted NCBI (registered with the BioProject ID: PRJNA739261 (http://www.ncbi.nlm.nih.gov/bioproject/739261). It is noteworthy, a total of 147 transcripts with length below 200 bp were removed during NCBI submission. Ce_assembly_unique_supertranscript.fasta: The corresponding supertranscript file constructed from the de novo transcriptome assembly of C. edule. Ce_assembly_unique.gff: The corresponding .gff file obtained from the de novo transcriptome assembly of C. edule. transcript_to_gene_map.txt: The corresponding transcripts-to-gene-mapping file obtained from the de novo transcriptome assembly of C. edule, and used for clustering analyses. rna_seq_de_novo_assembly_trinity_output.pdf: The resulting output file containing the statistics summary of the de novo transcriptome assembly of C. edule. Ce_assembly_unique_clustering.fasta: The resulting clustering .fasta file from the de novo transcriptome assembly of C. edule, using CD-HIT 4.8.1, setting 0.9 as the Sequence Identity Threshold. cluster_distribution_[assembled_transcripts].pdf: The figure shows the clusters distribution of the assembled transcripts. clustering_results_assembled_transcripts.pdf: Summary of the clustering analyses using CD-HIT 4.8.1.本数据集涵盖论文《暴露于腹泻性贝类毒素（Diarrhetic Shellfish Toxins）季节性污染的鸟尾蛤（Cerastoderma edule）转录组特征》（Domínguez-Pérez, D. 等，2021年）中，由从头转录组组装（de novo transcriptome assembly）与聚类分析得到的.fasta及.gff格式文件。具体文件说明如下：1. Ce_assembly_unique.fasta：提交至NCBI前的鸟尾蛤从头转录组组装结果，由Trinity v2.10.0软件将4份样本（未暴露于腹泻性贝类毒素的鳃与消化腺组织，以及暴露组样本）合并为单一转录组得到。2. C.edule_assembly_submitted_ncbi.fasta：已提交至NCBI的鸟尾蛤从头转录组组装结果（BioProject编号：PRJNA739261，访问链接：http://www.ncbi.nlm.nih.gov/bioproject/739261）。值得注意的是，在NCBI提交流程中，共移除了147条长度低于200 bp的转录本。3. Ce_assembly_unique_supertranscript.fasta：由鸟尾蛤从头转录组组装得到的对应超级转录本（supertranscript）文件。4. Ce_assembly_unique.gff：鸟尾蛤从头转录组组装对应的.gff格式注释文件。5. transcript_to_gene_map.txt：鸟尾蛤从头转录组组装所得的转录本-基因映射文件，用于后续聚类分析。6. rna_seq_de_novo_assembly_trinity_output.pdf：包含鸟尾蛤从头转录组组装统计汇总信息的输出文件。7. Ce_assembly_unique_clustering.fasta：使用CD-HIT 4.8.1软件，以0.9为序列同一性阈值，对鸟尾蛤从头转录组组装结果进行聚类所得的.fasta格式聚类文件。8. cluster_distribution_[assembled_transcripts].pdf：展示组装转录本聚类分布情况的统计图。9. clustering_results_assembled_transcripts.pdf：使用CD-HIT 4.8.1完成的聚类分析结果汇总文件。