PTEN analysis results.

Immunofluorescence (IF) was performed on formalin-fixed paraffin-embedded tumor specimen to assess for expression of PTEN (10q23.31) using the previously described AQUA technique (see Materials & Methods). Methylation specific PCR was performed on bisulfite treated DNA using two primers sets (MSP1 and MSP2) targeted to the promoter region of PTEN. PTEN copy number status for each case is summarized from the array CGH results. Italicized and bolded are three cases (CH1, CH33, and CH36) showing loss of PTEN despite maintainence of normal PTEN copy number status. NP = Not performed. F = Technical failure. U = Unmethylated. M = Methylated. PTEN copy number status based on array CGH: 0 = Homozygous deletion, 1 = Hemizygous deletion, 2 = Copy number neutral.