Immune-Epithelial Interactions and Tissue Remodeling in the Inflammatory Respiratory Nasal Tract [scRNA-seq]

Chronic rhinosinusitis (CRS) is a common inflammatory disease of the nasal cavity and sinuses that affects millions of individuals worldwide. The complex pathophysiology of CRS remains poorly understood, with emerging evidence implicating diverse immune and epithelial cell types in the disease process. We employed single-cell RNA sequencing (scRNA-seq) in dissociated tissues to investigate the cellular and molecular heterogeneity of CRS with and without nasal polyps. Overall design: Samples from patients with CRS with and without nasal polyps and healthy controls were collected and analyzed using single-cell RNA sequencing (scRNA-seq).