Additional file 2: of Analysis of the diversity and tissue specificity of sucrose synthase genes in the long read transcriptome of sugarcane

Figure S1. Identification and sequence retrieval of SuSy transcripts from the SUGIT transcriptome reference database. Shown here is the reads mapping to the reference SuSy4 gene from sugarcane using large gap mapping tool in the CLC-WB v 10. Figure S2. Alignment of nucleotide sequences of the SUGIT-SuSy1 transcripts with the reference sequences using Geneious v.11. Reference genes used in the alignment are from Sorghum accession numbers NC_012870, XM_002465116; bottom with Zea mays NC_024467 and NM_001111941. Figure S3. Alignment of nucleotide sequences of the SUGIT-SuSy2 transcripts with the reference sequences using Geneious v.11. Reference genes for alignment is from Saccharum officinarum, accession number AY118266 and its mRNA and cDNA sequences. Figure S4. Alignment of nucleotide sequences of the SUGIT-SuSy4 transcripts with the reference sequences using Geneious v.11. Reference genes used for alignment are from Zea mays accession numbers NC_012870 and mRNA and cDNA sequences of accession XM_2465268. Figure S5. Alignment of nucleotide sequences of the SUGIT-SuSy7 transcripts with the reference sequences using Geneious v.11. Reference genes used are from Zea mays accession numbers NC_024463 and mRNA and cDNA sequences of accession number XM_008646897. Figure S6. Conserved exon-intron structure across the monocots species available in Phytozome. Using BLAST tool available in the GBrowse, the SuSy transcripts were annotated with Sorghum as the reference genome. Figure S7. a. In genotype KQ228 SuSy 1, 2 and 7 have significantly different expression between each of the tissue types, based on one -way ANOVA results p