Interleukin-10/CSF1R fusion protein rejuvenates the exhausted T cells for executing antitumor activity IV

Bulk RNA sequence (RNA-seq) has been used to explore the effect of IL10/CSF1R fusion protein modulation in tumor microenvironment. Four groups were analyzed which contain Ctrl, CSF1R, IL10 and BF10. MTC-Q1 oral cancer cells were orthotopic injection into C57BL/6J mice. tumor tissue has been isolated (~ 100 mm3) and total RNA were extracted by Trizol, followed by cDNA were sequenced by Illumina NovaSeq6000. Overall design: Examination of therapeutic modulation of IL10/CSF1R fusion protein within tumor microenvironment in mice cancer model. RNA-seq of tumor tissues from orthotopic cancer tissue after four dose treatments.