Anti-tumor activity of hMSCs PTX and SR4987PTX.

HMSCs and mouse SR4987 were used to study anti-tumor activity. C57BL/6 and Nude mice received s.c. B16 melanoma cells at concentration of 2×105 and 2×106/0.2 ml saline respectively. Nod/Scid mice were injected s.c. with a human prostate carcinoma cells DU145 at the concentration of 2×106/0.2 ml saline. HMSCs and SR4987 primed or not with PTX were mixed with TCs 5 minutes before injection at the ratio (MSCs/TCs) shown in the Table. Similarly TCs were also mixed with PTX free at 2.000 ng/ml 5 minutes before injection. Nude mice injected with hMSCs and B16 melanoma cells were sacrificed on day 14 and Nod/Scid mice treated with human DU145 and hMSCs were sacrificed 40 days after cells injection. C57BL/6 mice receiving syngenic SR4987 and B16 melanoma cells were sacrificed on day 32. For each group of mice, tumors were excised and weighed. Numbers in the table are the means of tumor weight (TW) ± standard deviation (SD). Tumor takes (TT) indicate the % of mice with tumor at the time of sacrifice.(°): two mice were tumor free at time of sacrifice.*p**pIn C57BL/6 model SR4987PTX vs SR4987 was not significant.