Supplementary material 3 from: Theissinger K, Kästel A, Elbrecht V, Makkonen J, Michiels S, Schmidt S, Allgeier S, Leese F, Brühl C (2018) Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wetlands. Metabarcoding and Metagenomics 2: e21060. https://doi.org/10.3897/mbmg.2.21060

We pooled the library according to the number of specimens per sample and could show that our read abundance highly correlates with specimen abundance. Thus, we could use the read abundancies as surrogates for relative species abundancies.