Comparison of wild-type Synechocystis sp. PCC 6803 with strains overexpressing wild-type or 5'-sensing-deficient RNase E

We compared transcriptomic changes, 5'-triphosphorylated (TSS) and 5'-monophosphorylated (PSS) RNA ends of different strains of the cyanobacterium Synechocystis sp. PCC6803. Comparison encompassed wild-type Synechocystis (WT), a strain overexpressing RNase E and RNase HII (rne(WT)) and a strain overexpressing 5'-sensing-deficient RNase E and RNase HII (rne(5p)). Analysis of changing 5'-monophosphorylated ends revealed 5' sensing depedent processing sites on a transcriptome-wide level. Overall design: Triplicates of RNA samples of WT and rne(WT) as well as duplicates of rne(5p) grown at 30°C were analysed