Gregory Antipa (San Francisco State University) (2011) CIL:25687, Trichodina, Chlorella, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

Cytoplasm of a green Trichodina that lives on the surface of a freshwater snail collected in central Illinois. In this view the abundant endosymbiotic zoochlorellae and a single endosymbiotic bacterium are evident. The bacterial endosymbiont is filled with virion particles. In this image the particles can also be seen loose in the cytoplasm of Trichodina. See serial section CIL:25688). TEM taken by G. Antipa in 1968 on a Hitachi HU11A operating at 75kV. The negative magnification is 11,500X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

本数据集呈现了生活在伊利诺伊州中部淡水螺表面的一种绿色锥虫的细胞质。在此视角下，丰富的共生藻类 zoochlorellae 以及单个共生细菌清晰可见。细菌性内共生体中充满了病毒颗粒。在图像中，颗粒亦可见于锥虫细胞质之中。详见序列切片 CIL:25688。1968年由G. Antipa使用75kV的日立 HU11A 透射电子显微镜拍摄。负放大倍数为11,500倍。原始胶片由Epson Perfection V750 Pro 扫描。该图像适用于定量分析。采用标准戊二醛固定，随后以四氧化锇处理，经酒精脱水，并以环氧树脂包埋。微切片厚度约为65纳米。