supplemental data: Genes and pathways of regulatory T cells regulated by adenosine A2A receptor: a bioinformatics study.

The microarray data was analyzed by R. First, grey-scale map of chip data, normalized unscaled standard errors (NUSE) box graph, RNA degradation map and MA plot were used to evaluate the quality of the data by affyPLM, RColorBrewer, affy and oligo packages of R software. Second, the data was preprocessed by RMA method and GPL1261 platform was used to convert the probe ID to gene symbol. The missing values was supplemented by k-Nearest Neighbor (KNN). Last, the DEGs among groups were analyzed by limma packages of R. The criterions for DEGs were adjusted P-value < 0.05 and | log2fold-change(FC) | ≥1.