NOMe-Seq analysis of IMR-90 cell line II

NOMe-Seq analysis was performed with GC-recognizing cytosine DNA methyltransferase The IMR-90 nuclei were prepared with a solution containing 0.1% NP-40. Then, the nuclei were suspended in methylation buffer, and cytosine C-5 DNA methyltransferase recognizing GC dinucleotide was added. The solution was incubated at 37°C for 1 hr and collected nuclei. Then genomic DNA was extracted, and library preparation was performed with tPBAT protocol (Miura F et al., NAR, 2019)