DNA methylation analysis in MCF-7 cell line with overexpression of POU1F1

The transcription factor POU1F1 plays an important role in breast cancer. It has been described that POU1F1 is capable of regulating the expression of different genes involved in cell proliferation, apoptosis and cell plasticity. The mechanism by which POU1F1 carries out these regulations is still unknown, and epigenetics is one of the possible causes. Epigenetics refers to modifications in gene expression that are heritable through cell division and that occur without changes in the DNA sequence. DNA methylation is one of the most important epigenetic mechanisms. Analysis of DNA methylations using MCF-7 cells lines with overexpression of POU1F1