Unraveling independent origins of two tetraploid Achillea species by amplicon sequencing

Allopolyploidy is a significant mechanism of plant speciation, and many allopolyploid species have arisen recurrently. However, the probability that allopolyploidization between the same two parental species may lead to the origin of different taxa has received little attention. Here we used a new progenitor-specific amplicon sequencing method to demonstrate the independent origins of two yarrow species, Achillea alpina and A. wilsoniana, via allotetraploidy from the same diploid progenitor species pair, A. acuminata and A. asiatica. Based on the sequences of 17 nuclear genes from 21 wild populations of the four Achillea species investigated, a clear view of  genetic structure and demographic history was obtained with each species. Significant genetic differentiation was evident between the two tetraploid species. Two genetically distinguishable groups were detected within one of the progenitor, A. acuminata, and ancestors belonging to those two groups contributed to the two tetraploid ...