Exploiting Mouse Models to Understand Female Hypersensitivity to Cocaine. Exploiting Mouse Models to Understand Female Hypersensitivity to Cocaine

Male mice, female mice, ovariectomized female mice given placebo pellets, and ovariectomized female mice given extradiol pellets were treated with experimenter-administered saline or cocaine for 7 days, and behaviorally monitored. After a 28 day withdrawal period, RNA samples were prepared from nucleus accumbens and subjected to RNA-Seq, followed by differential expression analyses using Cuffdiff, Limma and DESeq2. Selected transcripts were further investigated using quantitative polymerase chain reaction. Overall design: After surgeries and non-operated controls, mice were administered saline or cocaine, and behaviorally analyzed. After withdrawal from drugs, nucleus accumbens RNA was used for RNA-Seq and qPCR analyses.