16S rRNA gene amplicon sequencing of lithoautotrophic iron(II)-oxidizing culture from an organic-rich paddy soil originating from Huilongpu (China)

Microbial nitrate reduction coupled to iron(II) oxidation (NRFeOx) plays a major role in paddy soils due to high levels of dissolved Fe(II) and regular application of nitrogen fertilizer. We successfully enriched a lithoautotrophic NRFeOx culture from a paddy soil (China, Hunan province), which is dominated by Gallionella (71%). The novel enrichment culture is providing the basis to study mechanisms of autotrophic NRFeOx and its impact on metal mobility and N2O emissions in paddy soils. Soil was sampled from the upper 20 cm were collected in September 2020 from a rice paddy field in Huilongpu, Hunan province, China. Microbial enrichments for autotrophic nitrate-reducing, iron(II)-oxidizing microorganisms were set up in April 2021 as decribed in the accompanied publication. Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region, and subsequently library preparation with Nextera (Illumina) 2nd step PCR and sequencing on Illumina MiSeq, v2, 2x250 bp were performed by Microsynth AG (Switzerland).