AP4 regulates stem and Paneth cell homeostasis and promotes adenoma initiation in the intestine (small intestinal organoid). Mus musculus

AP4 is frequently expressed in primary CRCs. However, the in vivo relevance of AP4 for development of intestinal tumor formation has not been analyzed by genetic approaches. ApcMin/+ mice with deletion of AP4 were generated and analyzed. The mRNA expression profiles of intestinal adenomas with and without functional AP4 were compared. Overall design: AP4 deficient ApcMin/+ mice and wild-type ApcMin/+ mice were sacrificed at 16 weeks of age. 3 tumor RNA samples were obtained for each genotype; each tumor RNA sample represented a pool of 5 tumors isolated from the same mouse.