A high-content arrayed CRISPR screen reveals genetic requirements for dynein-based trafficking

The RNA-binding protein SUGP1 was one of many proteins identified in a CRISPR-based screen for proteins that promote dynein-based cargo trafficking in U-2 OS cells. To elucidate how SUGP1 promotes transport, we investigated changes in gene expression caused by disrupting the SUGP1 gene in this cell line with CRISPR/Cas9. As controls, we disrupted the XCR1 gene, which is not expressed in U-2 OS cells, and treated cells with crRNAs that lack specific targets in the human genome. Sequences were quality trimmed by the commercial provider of the RNA-seq data before being provided to the contributors. Overall design: 9 RNA-seq datasets for 3 biological replicates for each crRNA condition (SUGP1, XCR1 and non-targeting control (NTC)).