Data for: Walnut PR10/Bet v1-like proteins interact with chitinase in response to anthracnose stress

Walnut is a significant woody oil tree species that has been increasingly
affected by anthracnose in recent years. Effective anthracnose control is
crucial for walnut yield and quality, which requires a comprehensive
understanding of the response mechanisms to Colletotrichum
gloeosporioides. The PR10/Bet v1-like proteins are involved in defense
against various diseases, therefore, in this study, 7 JrBet v1s
were identified from the walnut transcriptome (named JrBet v1-1~1-7),
whose open reading frame (ORF) was 414~483 bp in length with isoelectric
point ranging from 4.96 to 6.11. These JrBet v1s were classified into
three groups, with the MLP/RRP and Dicot PR-10 subfamilies each comprising
three members (the largest ones), indicating that the proteins within
these two subfamilies may have evolved from a shared ancestral gene within
the broader PR10/Bet v1 protein family. The cis-elements in the promoters
of JrBet v1s were involved in response to hormones, coercive, and plant
growth metabolism. Most JrBet v1s could be significantly upregulated by
walnut anthracnose, JrBet v1-1, JrBet v1-2, JrBet v1-4, and JrBet v1-6
peaked at 12 days of anthracnose stress, showing a 2.85- to 63.12-fold
increase compared to the control, while JrBet v1-3, JrBet v1-5 and JrBet
v1-7 peaked at 9 days, with a 3.23- to 27.67-fold increase. Furthermore,
the significant correlation of the upregulation under anthracnose stress
of JrBet v1s and JrChit02-1 as well as JrChit02-2, the genes encoding
chitinase, suggesting that during the long process of microevolution in
walnut-C. gloeosporioides interactions, walnut has developed a Bet
v1-chitinase defense mechanism to counteract pathogen invasion.