Characterization of triacylglycerol secretion with shikonin derivatives in Lithospermum erythrorhizon

Followings are explanations to the Excel files. Excel file name: result03_positive_181119_Yazaki_Lab_5 Analysis of TAG molecules secreted by L. erythrorhizon cells cultured under three different conditions; LS medium (dark), M9 medium (dark), and M9 medium (light). Lipids of plant cells were extracted into three parts, i.e., medium, cell surface, and the rest of the cell fraction. Abbreviations of each lipid molecule is as follows. PC, phosphatidylcholine; lysoPC, lysophosphatidylcholine; PE, phosphatidylethanolamine; lysoPE, lysophosphatidylethanolamine; PG, phosphatidylglycerol; PI, phosphatidylinositol; SQDG, sulfoquinovosyldiacylglycerol; MGDG, monogalactosyldiacylglycerol; DGDG, digalactosyldiacylglycerol; TAG, triacylglycerol; DAG, diacylglycerol; Acyl-ACP, acyl-acyl carrier protein; FAS, fatty acid synthase. Excel file name: GC-FID_rawdata-processed.xlsx Quantitative analysis of TAG in three fractions of L. erythrorhizon cells (T-TOM): medium, cell surface, and inside cells. L. erythrorhizon cells were cultured under three different conditions, i. e., LS medium (dark), M9 medium (dark) and M9 medium (light). Quantitative analysis of TAG in hairy root of L. erythrorhizon (hairy root), tobacco cultured cells (BY-2) and intact tobacco plant (tabacum) are given in separate sheets. Fatty acid composition of TAG recovered from three fractions (medium, cell surface, and inside cells) of L. erythrorhizon cells is also shown. Excel file name: lipidome_data_processed.xlsx Data of lipidome analysis by LC-MS are summarized. Three fractions of L. erythrorhizon cells (T-TOM): medium, cell surface, and inside cells were analyzed, and those cells were cultured under three different conditions; LS medium (dark), M9 medium (dark), and M9 medium (light). The data of each lipid molecule are given in separate sheets.