The oxytocin-prostaglandins pathways in the horse (Equus caballus) placenta during pregnancy, physiological parturition, and parturition with fetal membrane retention

For relative quantification of mRNA, we used RT-qPCR. For relative quantification of oxytocin peptide and oxytocin receptor protein, we used western blotting with densitometry. To quantify prostaglandin E2 and PGF2α content, we used enzyme immunoassays.