LINE1 elements at Distal Junctions of rDNA repeats regulate nucleolar organization in human embryonic stem cells [RNA-seq]

In this study, we investigated whether RNA Pol II-mediated gene expression is affected by repression of DJ-LINE1. Guide RNAs targeting DJ-LINE1 or control genomic regions were transfected for 48h in CRISPRi naïve hESC. Naive H9 hES cells were maintained RSeT. Cells were transfected with gRNA plasmids in RSeT media added with 10 nM DZNep and 5nM TSA (RSeT+DT). 48 hours later, cells were directly processed for RNA extraction and RNA-seq library prep.