Expression data from germinal center (GC) B cells of Tnfrsf13B wild type, knockout and A144E mutant mice 10 days post immunization with allogenic cells

B cells in the germinal center of the spleen produce antibodies in responses to different antigens. Tnfrsf13b knockout and A144E mice can underlie more aggressive B cells effector responses, possibly at the cost of inadvertent pathogenicity; however, neither the amplitude nor the pathogenicity are simple functions of the amount of antigen-specific IgG produced. We used microarrays to detail the global program of gene expression of germinal center B cells and identified distinct classes of up-regulated genes in the different strains of mice with Tnfrsf13b wild type, knockout, monoallilic or biallec A144E missense mutation. C57BL/6 background mice with wild type Tnfrsf13b, Tnfrsf13b-KO or harboring monoallelic (A144E-WT) or biallelic (A144E-A144E) Tnfrsf13b A144E mutations were immunized for 10 days via intraperitoneal injection of 50 million BALB/c splenocytes and thymocytes in PBS. Splenocytes were stained with FVS780, anti-CD19 APC, GL7 PE and CD95 FITC and germinal center B cells were sorted and the RNA was extracted for gene expression analysis via microarray.