Time lapse of increasing calcein fluorescence in the extracellular calcifying medium of the coral Stylophora pistillata following addition of calcein to seawater

Half-time of calcein influx was measured in the coral Stylophora pistillata as a method to investigate paracellular transport. To calculate half-life of calcein influx, time lapses of calcein influx into the extracellular calcifying medium was captured using confocal microscopy. The time lapse data set associated with figure 2 was recorded at 25 degrees in a seawater pH of 8. The study was carried on microcolonies of S. pistillata on glass coverslips. The study was conducted the Centre Scientifque de Monaco between 2016 and 2019.